10 April 2006

Two old biology papers

The effect of surgical wounding on tumour development Eur. J. Surg. Oncol. 1999 25:231-243 KEYWORDS: biology, wound-healing, extra-cellular matrix, ECM, hypoxia, hypoxic VOCAB: cytokine, autochthonous (originating where it is found), stroma (connective tissue existing for support alone), mitogen (a chemical that triggers mitosis, i.e. cell division) NOTES: -growth factors: EGF (epidermal growth factor), TGF-a (transforming growth factor), TGF-b, bFGF (basic FGF), IGF I (insulin-like growth factor), IGF II, PDGF (platelet-derived growth factor) -wounding stimulates some cell lines (How? Via what factors?) -wound healing stages: inflammatory, proliferative, repair & remodeling -“wound macrophages phagocytose wound debris” and trigger the next stage (proliferation) -“fibroblasts produce many of the extracellular matrix (ECM) components that result in scar tissue formation” -p53 is a tumor-suppressor -fibrinogen (via transglutamation) --> cross-linked fibrin -kDa = 1.1(residue count)/10 -bFGF (basic FGF) is highly angiogenic -this article considers treats as hypoxic concentrations below 50 ppm oxygen (presumably in medium) -ECM components: fibronectin, laminin, collagen, vitronectin, fibrinogen, thromospondin

Oxygen Tension Regulates the Expression of Angiogenesis Factor by Macrophages Science 23 Sept 1983 KEYWORDS: biology, wound-healing, angiogenesis, hypoxia VOCAB: debridement, Permanox culture dishes, lactalbumin hydrolysate, mitogen, mitogenic NOTES: -“sealed culture chambers”? -oxygen concentration in atmosphere reported -cultured cells at 20%, 10%, 5%, 2%, 0% oxygen in air --> corresponds to what in media? are some of these points saturated/otherwise indistinguishable? -“near-anoxia” = “sealed” chamber is not so sealed?


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